Description

Collects read counts at specified intervals. The count for each interval is calculated by counting the number of read starts that lie in the interval.

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

input

:file

BAM/CRAM/SAM file

*.{bam,cram,sam}

input_index

:file

BAM/CRAM/SAM index file

*.{bai,crai,sai}

intervals

:file

A file containing the specified intervals

*.{bed,intervals}

meta2

:map

Groovy Map containing reference information e.g. [ id:‘test’ ]

fasta

:file

Optional - Reference FASTA

*.{fasta,fa}

meta3

:map

Groovy Map containing reference information e.g. [ id:‘test’ ]

fai

:file

Optional - Index of the reference FASTA file

*.fai

meta4

:map

Groovy Map containing reference information e.g. [ id:‘test’ ]

dict

:file

Optional - Sequence dictionary of the reference FASTA file

*.dict

Output

name:type
description
pattern

hdf5

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.hdf5

:file

The read counts in hdf5 format

*.hdf5

tsv

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.tsv

:file

The read counts in TSV format

*.tsv

versions_gatk4

${task.process}

:string

The name of the process

gatk4

:string

The name of the tool

gatk --version | sed -n '/GATK.*v/s/.*v//p'

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

gatk4

:string

The name of the tool

gatk --version | sed -n '/GATK.*v/s/.*v//p'

:eval

The expression to obtain the version of the tool

Tools

gatk4
Apache-2.0

Genome Analysis Toolkit (GATK4). Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size.